Overexpression of resistin affect 3T3-L1 adipocyte lipid metabolism / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 251-255, 2007.
Article
in Zh
| WPRIM
| ID: wpr-247341
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of resistin overexpression on 3T3-L1 adipocyte lipid and glucose metabolism.</p><p><b>METHODS</b>Expression vector for rat resistin gene was constructed and transfected into 3T3-L1 adipocytes. Cell differentiation and lipid accumulation was determined by Oil Red O staining. Differentiation marker genes (pref-1, C/EBPalpha, FAS) and glucose transporter 4 (GLUT4) gene mRNA expressions were evaluated by reverse transcription-PCR (RT-PCR). Triglyceride (TG) and free fatty acids (FFAs) in adipocytes were measured by colorimetric kit.</p><p><b>RESULTS</b>(1) In resistin-overexpressed adipocytes, the lipid droplets presented at the second day which was earlier than the control cells. (2) The expression of C/EBPalpha and FAS genes in resistin-overexpressed adipocytes were up-regulated and the pref-1 was down-regulated compared with that of the control cells. (3) In resistin-overexpressed adipocytes, cellular TG and FFAs levels were significantly increased (P<0.05). (4) There was no difference in the expression of GLUT4 gene between 3T3-L1 adipocytes and resistin-overexpressed adipocytes (P> 0.05).</p><p><b>CONCLUSION</b>Overexpression of resistin can affect 3T3-L1 adipocyte lipid metabolism and thereby result in obesity and insulin resistance, but have no effect on GLUT4 gene expression.</p>
Full text:
1
Index:
WPRIM
Main subject:
Triglycerides
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Gene Expression
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Cell Differentiation
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Adipocytes
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3T3-L1 Cells
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Resistin
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Glucose Transporter Type 4
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Lipid Metabolism
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Fatty Acids, Nonesterified
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Genetics
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Medical Genetics
Year:
2007
Type:
Article