Study on the mitochondrial DNA variation in patients with type 2 diabetes mellitus / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 167-172, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-247361
ABSTRACT
<p><b>OBJECTIVE</b>To explore the relationship between type 2 diabetes mellitus (T2DM) and the mutations in the fragment of mitochondrial DNA (mtDNA) from nucleotides 3153 to 3551, which have shown high frequency of point mutation.</p><p><b>METHODS</b>One hundred and ninety-one normal controls and 222 patients with T2DM were screened by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), T-A cloning sequencing and denatured high performance liquid chromatography (DHPLC) techniques.</p><p><b>RESULTS</b>The prevalence of mtDNA mutations in the patient group (24.32%) was significantly higher than that in the control group (7.33%) (P < 0.05). Three novel mutations of A3209T, T3253G and A3467C were found, and C3497T was first reported in DM. Onset age, body mass index, fasting blood glucose, HbA1C, high density lipoprotein-cholesterol and diabetic nephropathy could be related to occurrence of mtDNA mutations (P < 0.05).</p><p><b>CONCLUSION</b>Mitochondrial DNA mutations might implicate T2DM in Wenzhou population, which should play an important role in the pathogenesis of T2DM.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymorphism, Restriction Fragment Length
/
DNA, Mitochondrial
/
DNA Mutational Analysis
/
Polymerase Chain Reaction
/
Chromatography, High Pressure Liquid
/
Diabetes Mellitus, Type 2
/
Genetics
/
Mutation
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2007
Type:
Article
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