Simultaneous determination of four active compounds in tiangou capsules by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a method for quantitative analysis of gastrodin, baicalin, paeonolum and pinoresinol diglucoside in Tiangou capsules by HPLC.</p><p><b>METHOD</b>The separation was performed on SinoChrom ODS-BP column (4.6 mm x 150 mm, 5 microm). The mobile phase consisted of acetonitrile and 0.2% phosphoric acid aqueous solution with gradient elution program. The flow rate was 1.0 ml x min(-1) and the column temperature was 30 degrees C. UV detection wavelength was set at 230 nm.</p><p><b>RESULT</b>The linear ranges of gastrodin, baicalin, paeonolum and pinoresinol diglucoside were 0.1168-1.1680, 0.1142-1.1420, 0.0512-0.5120, 0.0556-0.5560 microg, respectively. The average recoveries of gastrodin, baicalin, paeonolum and pinoresinol diglucoside were 100.3% (RSD 0.53%), 99.96% (RSD 1.15%), 99.90% (RSD 1.17%), 99.97% (RSD 1.62%), respectively.</p><p><b>CONCLUSION</b>The method is accurate, simple, feasible and reliable, and is available for the quality control of Tiangou capsules.</p>