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Study on discriminating nephrotoxic components in Zexie / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 758-761, 2011.
Article in Chinese | WPRIM | ID: wpr-247391
ABSTRACT
<p><b>OBJECTIVE</b>To sift and identify the nephrotoxic components in Zexie for controlling the quality of the herb.</p><p><b>METHOD</b>The fractions of zexie were prepared by Pre-HPLC, then the nephrotoxicity of the fraction was sifted using LLC-PK1 labelled with fluorescein diacetate and MTT assay. Finally, the compounds in the most obvious nephrotoxic fraction were identified with LC-MS.</p><p><b>RESULT</b>Using MTT and FDA assay, similar results were obtained. Fraction C13 was found to be the most toxic with FDA assay, in which three compounds, alisol C, 16, 23-oxido-alisol B and alisol O, were detected and characterized by multi -stage mass spectrometric analysis.</p><p><b>CONCLUSION</b>Alisol C, 16, 23-oxido-alisol B and alisol O in Zexie may cause nephrotoxicity.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Mass Spectrometry / Swine / Chemistry / Chromatography, High Pressure Liquid / LLC-PK1 Cells / Alisma / Toxicity / Kidney Type of study: Prognostic study Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2011 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Mass Spectrometry / Swine / Chemistry / Chromatography, High Pressure Liquid / LLC-PK1 Cells / Alisma / Toxicity / Kidney Type of study: Prognostic study Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2011 Type: Article