Study on discriminating nephrotoxic components in Zexie / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 758-761, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-247391
ABSTRACT
<p><b>OBJECTIVE</b>To sift and identify the nephrotoxic components in Zexie for controlling the quality of the herb.</p><p><b>METHOD</b>The fractions of zexie were prepared by Pre-HPLC, then the nephrotoxicity of the fraction was sifted using LLC-PK1 labelled with fluorescein diacetate and MTT assay. Finally, the compounds in the most obvious nephrotoxic fraction were identified with LC-MS.</p><p><b>RESULT</b>Using MTT and FDA assay, similar results were obtained. Fraction C13 was found to be the most toxic with FDA assay, in which three compounds, alisol C, 16, 23-oxido-alisol B and alisol O, were detected and characterized by multi -stage mass spectrometric analysis.</p><p><b>CONCLUSION</b>Alisol C, 16, 23-oxido-alisol B and alisol O in Zexie may cause nephrotoxicity.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Mass Spectrometry
/
Swine
/
Chemistry
/
Chromatography, High Pressure Liquid
/
LLC-PK1 Cells
/
Alisma
/
Toxicity
/
Kidney
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2011
Type:
Article
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