Optimization of the hydrolysis process for C21 steroidal glycoside of bai shou wu by acetic acid with multi-target orthogonal design / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the optimizal hydrolysis process for C21 steroidal glycoside of Bai Shou Wu by acetic acid.</p><p><b>METHOD</b>The effects of acetic acid concentration, reaction temperature and reaction time had been investigated using orthogonal design and the contents of kidjoranin 3-O-beta-digitoxopyranoside, caudatin, kidjoranin 3-O-alpha-L-diginopyranosyl-(1 --> 4)-beta-cymaropyranoside and caudatin 3-O-beta-cymaropyranoside as response indexs were determined by the high performance liquid chromatography.</p><p><b>RESULT</b>The factors influencing acetic extraction efficiency were as follows A > B > C (A. reaction temperature; B. reaction time; C. acetic acid concentration). The optimal hydrolysis condition obtained was refluxing for 6 hours with 5.0% dilute CH3COOH solution at 100 degrees C.</p><p><b>CONCLUSION</b>The content of antitumor active ingredients under the optimum hydrolysis condition is raised obviously and has a great part in studying this antitumor drug.</p>