Hydrolysis technology optimization of phorbol esters by orthogonal experiment / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 446-449, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-247461
ABSTRACT
<p><b>OBJECTIVE</b>To establish the best hydrolytic conditions from phorbol esters.</p><p><b>METHOD</b>The orthogonal experiment was used to optimize 4 factors, which were reaction time, ratio of solid-to-liquid, hydrolytic times, and temperature. Diamonsil C18 column (4. 6 mm x 250 mm, 5 microm) was used and the mobile phase was consisted of acetonitrile and water for HPLC detection. The detection wavelength was set at 234 nm, the flow rate was 1 mL x min(-1), and the column temperature was 25 degrees C.</p><p><b>RESULT</b>The optimum conditions were 10 h of reaction time, 16 of solid-to-liquid (BaOH/MeOH) ratio, 25 degrees C of temperature, and one time of hydrolysis. There was a good linear relationship of phorbol in the range of 4.28-107 mg x L(-1) (r = 0.999 9), and the average recovery was 97.89%, with RSD 0.78%.</p><p><b>CONCLUSION</b>The method is steady, reliable and reproducible, and it provides a mean for future study.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phorbol Esters
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Hydrolysis
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2011
Type:
Article
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