Study on the correlation of interleukin-13 polymorphism and susceptibility to chronic obstructive pulmonary disease in Chinese Han population / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 607-611, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-247509
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the polymorphism of interleukin (IL)-13 gene in Chinese Han population, and to study the possible association of IL-13 polymorphism and the susceptibility to chronic obstructive pulmonary disease (COPD).</p><p><b>METHODS</b>Using genomic DNA extracted with phenolchloroformisoamyl alcohol from the blood of 94 healthy smokers and 88 COPD smokers, three single nucleotide polymorphism (SNP) sites in IL-13 gene marked as 1103C/T, 4257G/A, 4738G/A were determined by polymerase chain reaction/restriction fragment length polymorphism analysis or polymerase chain reaction/single strand conformation analysis. In addition, plasma IL-13 concentration was measured by ELISA on 24 healthy smokers and 24 smokers with chronic obstructive pulmonary disease.</p><p><b>RESULTS</b>(1) To 4257G/A and 4738G/A SNP sites, the genotype frequencies and allele frequencies were not significantly different between healthy smokers and COPD smokers (P > 0.1). (2) To 1103C/T SNP site, relative higher CC genotype frequency and C allele frequency were found in healthy smokers (P < 0.05), also C allele could prevent smokers from chronic obstructive pulmonary disease, with OR 0.56 and 95% confidence interval was 0.34 - 0.91. (3) Plasma IL-13 concentration was not affected by genotype of 4257G/A or 4738G/A SNP sites. However, 1103CC showed for significantly lower IL-13 concentration in plasma (P < 0.05).</p><p><b>CONCLUSION</b>The genotype of IL-13 1103C/T SNP site was associated with the susceptibility to COPD in Chinese Han population and 1103CC accounted for relative lower plasma IL-13 concentration and lower risk for COPD.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymorphism, Genetic
/
Blood
/
Enzyme-Linked Immunosorbent Assay
/
Ethnicity
/
Risk Factors
/
Interleukin-13
/
Genetic Predisposition to Disease
/
Polymorphism, Single Nucleotide
/
Pulmonary Disease, Chronic Obstructive
/
Genetics
Type of study:
Etiology study
/
Risk factors
Limits:
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Epidemiology
Year:
2004
Type:
Article
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