Enhancement of antiviral immunity in HBV mouse model by blocking PD-1/PD-L1 signaling pathway / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 263-266, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-247540
ABSTRACT
<p><b>OBJECTIVE</b>To establish a mouse model for human chronic HBV infection, and to investigate the role of PD-1/PD-L1 signaling pathway in antiviral immunity.</p><p><b>METHODS</b>A mouse model was established by hydrodynamic injection of the plasmid pAAV/HBV1.2-GFP into the tail vein of C57BL/6 mice, HBV markers were assayed at different time points after injection. After intraperitoneal injection of anti-PD-L1 monoclonal antibody, the serum ALT, and HBV DNA in the serum, liver and kidney were assayed.</p><p><b>RESULTS</b>The chronic HBV infection mouse model were established successfully, serum HBsAg and high load of HBV DNA were detectable 90 days after plasmid injection. After blocking of the PD-1/PD-L1 pathway, the serum ALT level of mice were significantly increased (P < 0.01), and the HBV DNA load in serum (P < 0.01), liver (P < 0.05) and kidney (P < 0.05) were decreased significantly.</p><p><b>CONCLUSION</b>Blocking the PD-1/PD-L1 signaling pathway can enhance antiviral response in mice with chronic HBV infection.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Virology
/
DNA, Viral
/
Membrane Glycoproteins
/
Signal Transduction
/
Hepatitis B virus
/
B7-1 Antigen
/
Hepatitis B, Chronic
/
Disease Models, Animal
/
Allergy and Immunology
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2010
Type:
Article
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