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Characteristics of CDR3 of TCR beta on CD8+ T cells in chronic hepatitis B patients / 中华肝脏病杂志
Chinese Journal of Hepatology ; (12): 184-188, 2010.
Article in Chinese | WPRIM | ID: wpr-247561
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the characteristics of CDR3 of TCRbeta on CD8+ T cells in chronic hepatitis B patients.</p><p><b>METHODS</b>Eight patients with chronic hepatitis B (ALT more than 2 ULN) were enrolled in this study. CD8+ T cells were isolated from peripheral blood. RT-PCR was proformed to amplify the CDR3 of TCRbeta, and the PCR products were sequenced and analyzed.</p><p><b>RESULTS</b>The chronic hepatitis B patients showed obvious clonal expansion of T cell, and three perturbation patterns of T cell expansion were showed in the CDR3 of TCRbeta, including monoclonicity, oligoclonicity and skewed peak patterns. The number of perturbation families of CD8+ subpopulation was significantly higher than that of CD8- subpopulation (10.6+/-4.7 vs. 4.1+/-3.1, t = 6.619, P less than 0.01). In 3 out of 8 patients, the number of perturbation families of CD8+ subpopulation was also higher than that of PBMCs without depleting CD8+ subpopulation.</p><p><b>CONCLUSIONS</b>The characteristics of CDR3 of TCRbeta may help to understand the inflammatory response in CHB patients.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Blood / Humans / Male / Receptors, Antigen, T-Cell / CD8-Positive T-Lymphocytes / Adult / Hepatitis B, Chronic / Genes, T-Cell Receptor beta / Complementarity Determining Regions / Allergy and Immunology Language: Chinese Journal: Chinese Journal of Hepatology Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Blood / Humans / Male / Receptors, Antigen, T-Cell / CD8-Positive T-Lymphocytes / Adult / Hepatitis B, Chronic / Genes, T-Cell Receptor beta / Complementarity Determining Regions / Allergy and Immunology Language: Chinese Journal: Chinese Journal of Hepatology Year: 2010 Type: Article