Study on gene delivery via the bile duct to rat liver using naked DNA particles / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 140-143, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-247576
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the efficacy of gene delivery to the right lateral lobe of the rat liver via a branch of the bile duct using naked DNA.</p><p><b>METHODS</b>We evaluated regional gene delivery to the right lateral lobe of the rat liver via a branch of the bile duct, using naked DNA, including pGL3, pCMV beta and Cy3 labeled CMV beta.</p><p><b>RESULTS</b>Gene expression was observed in right lateral lobe of both the damaged and the normal rats liver. The gene delivery efficiency was similar in two groups (P > 0.05). Gene expression was found in the right lateral lobe of damaged and normal livers. Fluorescence was observed in the region of the portal triads, and occasionally, in the lobule.</p><p><b>CONCLUSION</b>Retrograde infusion of naked DNA via the bile duct is an effective way to deliver genes to in both damaged and normal rat liver.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Plasmids
/
Rats, Inbred Lew
/
Therapeutics
/
Bile Ducts
/
DNA
/
Genetic Therapy
/
Gene Expression
/
Beta-Galactosidase
/
Genes, Reporter
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2010
Type:
Article
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