The effects of different PAP domains on hepatitis B virus replication / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 105-108, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-247583
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of different PAP domains on hepatitis B virus replication.</p><p><b>METHODS</b>The full length and two truncated PAP mutants were cloned into a eukaryotic expression plasmid, and were transfected into HepG2.2.15 cells using lipofectamine 2000. 3 days after transfection, the medium and cells were collected. HBsAg and HBeAg were measured using ELISA. The titers of HBV DNA were quantified using fluorogenic quantitative PCR (FQ-PCR). HepG2 cells were used to determine the cytotoxicity of the plasmids transfection by MTT assays.</p><p><b>RESULTS</b>The inhibitory effect on HBV replication of the C-terminal 25 amino acids deleted PAP mutant (pXF3H-PAP14) was not significantly different from that of the full length PAP (pXF3H-PAP12) (Chi-square test = 0.5, 2.0, 0.02, probability value more than 0.05), however, the cytotoxicity of pXF3H-PAP14 was lower than that of pXF3H-PAP12 (Chi-square test = 7.7, probability value less than 0.01). Both N-terminal 69 amino acids deleted mutant and C-terminal 25 amino acids deleted mutant had no cytotoxicity and no antiviral activity.</p><p><b>CONCLUSION</b>C-terminal 25 amino acid of PAP is related to cytotoxicity but not related to antiviral activity of PAP. N-terminal 69 amino acid of PAP is related to the anti-HBV effect of PAP.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Antiviral Agents
/
Pharmacology
/
Physiology
/
Plasmids
/
Virus Replication
/
DNA, Viral
/
Transfection
/
Hepatitis B virus
/
Blotting, Western
/
Amino Acid Sequence
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2010
Type:
Article
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