Comparison of the Golgi proteome of hepatocellular carcinoma with that of the adjacent non-tumor tissues / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 23-26, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-247608
ABSTRACT
<p><b>OBJECTIVE</b>To compare the Golgi proteome of hepatocellular carcinoma (HCC) with that of the adjacent non-tumor tissues.</p><p><b>METHODS</b>Hepatocellular carcinoma and adjacent non-tumor tissues were obtained from HCC patients. The protein expression maps in Golgi were obtained by two-dimensional gel electrophoresis (2-DE), and the differentially expressed protein spots were analyzed by PD-Quest software. Peptide mass fingerprint (PMF) of differential protein spots was obtained with MALD-TOT-MS.</p><p><b>RESULTS</b>According to 2-DE maps, the average numbers of protein spots were (1153+/-49) and (1086+/-37) in hepatocellular carcinoma and the adjacent non-tumor tissues. Compared to the adjacent non-tumor tissues, 27 proteins were upregulated, and 20 proteins were downregulated in HCC Golgi.</p><p><b>CONCLUSIONS</b>The Golgi proteome in HCC tissues is different from that in the adjacent non-tumor tissues, and the differential expression proteins are involved in energy metabolism, tumor metastasis, and cell cycle regulation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Electrophoresis, Gel, Two-Dimensional
/
Annexin A5
/
Carcinoma, Hepatocellular
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Proteome
/
Golgi Apparatus
/
Liver
/
Liver Neoplasms
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2010
Type:
Article
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