Research on biological and genetic characteristics of human placenta mesenchymal stem cells cultured in vitro / 中华医学遗传学杂志
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
; (6): 471-475, 2016.
Article
in Zh
| WPRIM
| ID: wpr-247655
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the biological characteristics and genetic features of human placenta mesenchymal stem cells (hPA-MSCs) cultured in vitro in order to assess its safety for clinical use.</p><p><b>METHODS</b>The shapes of the 1st, 3rd, 5th, 7th, 10th, 13th, 17th and 20th generation hPA-MSCs cultured in vitro using serum-free culture medium were observed. Their cell cycle, cell surface markers, and karyotype were analyzed, and relevant genes and cytokines were measured.</p><p><b>RESULTS</b>The shape of hPA-MSCs has remained as fusiform or short fusiform, and there was no significant change. About 93% of hPA-MSCs cells were in G0/G1 phase and remained stable. No obvious chromosomal translocation, loss or inversion was noted by karyotyping analysis. Cytokines expression level remained stable. Related gene expression level as a whole was on the decline, but the gene expression level of the first five generations showed very slight variations, with genetic characteristics remaining stable.</p><p><b>CONCLUSION</b>The hPA-MSCs cultured in vitro with serum-free medium has retained stable in the first five generations.</p>
Full text:
1
Index:
WPRIM
Main subject:
Physiology
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Placenta
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Cells, Cultured
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Cytokines
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Cell Biology
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Mesenchymal Stem Cells
/
Karyotyping
Limits:
Female
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Humans
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Pregnancy
Language:
Zh
Journal:
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
Year:
2016
Type:
Article