Seminal plasma miR-122-3p and miR-141-5p stability and its diagnosis value for idiopathic asthenospermia / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 320-323, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-247682
ABSTRACT
<p><b>OBJECTIVE</b>To explore the stability of seminal plasma miR-122-3p and miR-141-5p and their diagnostic value for idiopathic asthenospermia.</p><p><b>METHODS</b>Seminal plasma miR-122-3p and miR-141-5p with various incubation time, freeze-thaw cycles and incubation time were analyzed. Real-time fluorescence quantitative polymerase chain reaction (RT-PCR) was used to detect the content of miR-122-3p and miR-141-5p, with U6 snRNA as the reference. The idiopathic asthenospermia group was further divided into grade I and II based on the progressive motility percent, and the content of miR-122-3p and miR-141-5p in the two grades were compared.</p><p><b>RESULTS</b>No difference was detected after incubation at room temperature and 4 degrees Celsius. But seminal plasma miR-122-3p and miR-141-5p have decreased along with the increase in freeze-thaw cycles increases. RT-PCR assayed showed that the miR-122-3p content in the idiopathic asthenospermia group was significantly lower than the control group, while miR-141-5p was significantly higher. The difference in both miR-122-3p and miR-141-5p content was statistically significant (P < 0.05). A significant difference in miR-122-3p and miR-141-5p content between a and b groups was also detected (P < 0.05). The AUC-ROC of miR-122-3p was 0.88. At the cutoff value of 1.02, the sensitivity and specificity was 83% and 84%, respectively. At the cutoff of 2.95, the AUC-ROC of miR-141-5p was 0.88. The sensitivity and specificity was 84% and 70%, respectively.</p><p><b>CONCLUSION</b>MiR-122-3p and miR-141-5p in seminal plasma are stable and have certain value for the diagnosis of idiopathic asthenospermia.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Semen
/
Chemistry
/
ROC Curve
/
MicroRNAs
/
Diagnosis
/
Asthenozoospermia
/
Real-Time Polymerase Chain Reaction
/
Genetics
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Adult
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Medical Genetics
Year:
2016
Type:
Article
Similar
MEDLINE
...
LILACS
LIS