Matrix metalloproteinase 8 and 9 regulations of polymorphonuclear leukocytes stimulated by Porphyromonas gingivalis with different fimA genotypes / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 206-209, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-248271
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the pathogenicity of matrix metalloproteinase 8, 9 (MMP-8, MMP-9) regulations of polymorphonuclear leukocytes (PMNs) by challenge of Porphyromonas gingivalis (P. gingivalis) with different fimA genotypes.</p><p><b>METHODS</b>The studies mainly adopt the isopycnic sedimentation separation to separate the PMNs from human peripheral blood. P. gingivalis ATCC 33277 (type I), WCSP 115 (type II), WCSP 1.5 (type III), W83 (type IV), WCSP 559 (type IV) were assessed for their inductions of MMP-8, MMP-9 expression in PMNs. MMP-8, MMP-9 protein levels in culture supernatant were determined by ELISA at different time intervals (5 min, 30 min, 1 h, 2 h) following continuous co-culture of bacteria with PMNs.</p><p><b>RESULTS</b>MMP-8 and MMP-9 protein levels produced by PMNs co-culture with the I fimA-IV fimA P. gingivalis were significantly stronger than unsimulated group. The velocity and quantity of MMP-8 produced by PMNs co-culture with the II fimA P. gingivalis and IV fimA P. gingivalis were more than III fimA, IVfimA P. gingivalis. The MMP-9 protein levels produced by PMNs co-culture with the I fimA, II fimA, IV fimA P. gingivalis was significantly stronger than III fimA and IV fimA P. gingivalis.</p><p><b>CONCLUSION</b>II fimA and IV fimA P. gingivalis have stronger pathogenicity relatively, which indicate that fimA genotype is associated with pathogenesis of P. gingivalis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Porphyromonas gingivalis
/
Coculture Techniques
/
Matrix Metalloproteinase 8
/
Fimbriae Proteins
/
Genotype
/
Neutrophils
Limits:
Humans
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2009
Type:
Article
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