Muscarinic Acetylcholine Receptor Subtype Expression in Type Vestibular Hair Cells of Guinea Pigs / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 682-686, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-248603
ABSTRACT
Recent studies have demonstrated that five subtypes (M1-M5) of muscarinic acetylcholine receptor (mAChR) are expressed in the vestibular periphery.However,the exact cellular location of the mAChRs is not clear.In this study,we investigated whether there is the expression of M1-M5 muscarinic receptor mRNA in isolated type Ⅱ vestibular hair cells of guinea pig by using single-cell RT-PCR.In vestibular end-organ,cDNA of the expected size was obtained by RT-PCR.Moreover,mRNA was identified by RT-PCR from individually isolated type Ⅱ vestibular hair cells (single-cell RT-PCR).Sequence analysis confirmed that the products were M1-M5 mAChR.These results demonstrated that M1-M5 mAChR was expressed in the type Ⅱ vestibular hair cells of the guinea pig,which lends further support for the role of M1-M5 mAChR as a mediator of efferent cholinergic signalling pathway in vestibular hair cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Year:
2011
Type:
Article
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