Expression of HPV16 L1 protein in insect cell suspension culture system / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 352-354, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-248756
ABSTRACT
<p><b>OBJECTIVE</b>To express the L1 protein of human papillomavirus type 16 (HPV16) in insect cell suspension culture system.</p><p><b>METHODS</b>Optimized the conditions of suspension culture, recombinant virus amplification and protein expression. Determined the virus tilter by plague analysis and detected the target protein by SDS-PAGE and Western blot; The formation of VLPs by HPV16 L1 protein was observed with TEM.</p><p><b>RESULTS</b>The Sf9 cells could grow better in suspension culture with seeding density of 5 x 10-5 cell/mL and the maximum expression quantity was obtained by infection of cells with rBacV/HPV16L1 (MOI =10) and harvesting after 72-84 h. HPV16L1 protein could assemble into VLPs in Sf9 cells observed with TEM.</p><p><b>CONCLUSION</b>The conditions of cell culture, virus amplification and protein expression were optimized. HPV16 L1 protein could assemble into VLPs in Sf9 cells, which would provide a foundation for further study of the vaccine and diagnosis kits.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Suspensions
/
Virion
/
Recombinant Proteins
/
Oncogene Proteins, Viral
/
Spodoptera
/
Capsid Proteins
/
Cell Proliferation
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2007
Type:
Article
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