Co-expression of HSV-1 gD gene and interleukin-2 gene in dendritic cell / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 328-330, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-248764
ABSTRACT
<p><b>OBJECTIVE</b>To construct eukaryotic expression plasmid IRES-gD-IL-2 which contains both HSV-1 glycoprotein D (gD) gene and IL-2 gene and to induce it to express in antigen presenting cell (APC) -- dendritic cells (DCs).</p><p><b>METHODS</b>The whole sequence of gD and IL-2 were amplified by PCR assay. After confirmation by PCR, double-enzyme digestion and sequencing, these genes were directly cloned into eukaryotic expression vector IRES, then were transfected into DCs. Western blotting was employed to identify the transcription and expression of gD gene.</p><p><b>RESULTS</b>The results of PCR and enzyme digestion showed that the recombinant expression plasmid contained correct fragments, and the transcription and expression of gD were confirmed by Western blotting.</p><p><b>CONCLUSION</b>The recombinant expression vector IRES-gD-IL-2 was constructed, the results of the Western blotting showed that the recombinant protein could be identified by gD- specific antibody, therefore the protein has immunologic competence.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Dendritic Cells
/
Recombinant Fusion Proteins
/
Viral Envelope Proteins
/
Blotting, Western
/
Interleukin-2
/
Vaccines, DNA
/
Herpes Simplex Virus Vaccines
/
Allergy and Immunology
/
Genetics
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2007
Type:
Article
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