Detection the coinfection of Hantavirus and Orientia tsutsugamushi in primary cultured mite cells / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 307-309, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-248771
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether HV and Ot can coexist in their host (Leptotrombidium scutellare).</p><p><b>METHODS</b>Collecting the separate Leptotrombidium scutellare and the ones from mice in epidemic area. The cells of mites at larva, nymph, and adult stages were cultured and made into smear. In situ RT-PCR and PCR were used to detect and locate HV RNA and Ot DNA in the primary cultured cells.</p><p><b>RESULTS</b>Positive signals of HV RNA and Ot DNA distributed mostly in epithelial cells of digestive system and ovary cells of larva and nymph. The positive rate increased by the generation of passages.</p><p><b>CONCLUSION</b>Coinfection of HV and Ot did exist in wild Leptotrombidium scutellare.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Orientia tsutsugamushi
/
Virology
/
DNA, Bacterial
/
RNA, Viral
/
Cells, Cultured
/
Orthohantavirus
/
Reverse Transcriptase Polymerase Chain Reaction
/
Microbiology
/
Mites
Type of study:
Diagnostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2007
Type:
Article
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