The correlation of HBeAg expression and HBV-DNA in serum or peripheral blood mononuclear cells in patients with chronic hepatitis B / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 261-263, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-248784
ABSTRACT
<p><b>OBJECTIVE</b>To study the relationship between HBeAg expression and HBV-DNA in serum and peripheral blood mononuclear cells (PBMCs).</p><p><b>METHODS</b>208 patients with chronic hepatitis B were included in this present study. HBV-DNA in the PBMCs were performed by polymerase chain reaction (PCR), with the serum HBV-DNA level being determined by the way of fluoresces quantities PCR (FQ-PCR). Meanwhile, HBV-GM was also detected via enzyme-linked immunosorbent assay (ELISA).</p><p><b>RESULTS</b>There were 106 patients for positivity in the HBV-DNA level of PBMCs with 102 for negativity, in which the HBV-DNA high levels (HBV DNA load > or = 1.0E5) in serum were 91.5%, 45.1% (chi2=52.12, P>0.01) respectively, with 76.4% and 50.9% (chi2=21.55, P>0.01) for the positive percentage of HBeAg expression.</p><p><b>CONCLUSION</b>A significantly positive correlation was found between HBV-DNA in PBMCs and serum HBV-DNA along with the positive percentage of HBeAg, indicating that obvious PBMCs' increase infected by HBV in patients with positivity of HBeAg and high level of serum HBV-DNA.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
Blood
/
DNA, Viral
/
Enzyme-Linked Immunosorbent Assay
/
Leukocytes, Mononuclear
/
Hepatitis B, Chronic
/
Reverse Transcriptase Polymerase Chain Reaction
/
Allergy and Immunology
/
Genetics
/
Hepatitis B
Limits:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2007
Type:
Article
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