Immune responses induced by recombinant adenovirus Ad5F35-LMP2 in rhesus monkeys / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 226-228, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-248796
ABSTRACT
<p><b>OBJECTIVE</b>To observe the specific cellular and humoral immune responses after immunization with recombinant adenovirus Ad5F35-LMP2 in rhesus monkeys.</p><p><b>METHODS</b>Sixteen rhesuses were immunized with Ad5F35-LMP2 through intra-muscular injection in three groups high dosage group (1.5 x 10(10) TCID(50)/rhesus), medium dosage group (1.5 x 10(9)TCID(50)/rhesus), low dosage group (1.5 x 10(8)TCID50/rhesus) and the last group was control (PBS 4 ml/rhesus). They were totally immunized three times at intervals of one month. The EBV-LMP2 specific cellular immune responses were tested during the 0, 4, 8, 12 weeks by Elispot after immunization respectively. And the titers of anti-LMP2 antibody were tested by EIA at the same time.</p><p><b>RESULTS</b>EBV-LMP2 specific cellular and humoral immune responses which were induced by recombinant adenovirus Ad5F35-LMP2 can be found in all the three dosage groups. The potency of immune responses was related with the dosage of immunization. Higher dosage elicited more potent immune response.</p><p><b>CONCLUSION</b>The recombinant adenovirus Ad5F35-LMP2 could elicit LMP2 specific cellular and humoral immune responses in rhesus.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombinant Fusion Proteins
/
Cell Differentiation
/
Adenoviruses, Human
/
Viral Matrix Proteins
/
Immunization
/
Herpesvirus 4, Human
/
Allergy and Immunology
/
Genetics
/
Immunity, Cellular
/
Macaca mulatta
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2007
Type:
Article
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