Immunodominance in CD8+ T cell responses to HIV-1 synthesized epitopes / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 217-219, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-248799
ABSTRACT
<p><b>OBJECTIVE</b>To investigate immunodominance in CD8+ T cell responses to human immunodeficiency virus type 1 (HIV-1) epitopes.</p><p><b>METHODS</b>Frequency of Interferon-gamma (IFN-gamma) secreting cells and the proliferation percentage of CD8+ T cells in PBMC from an HIV-1-infected long term nonprogressor (LTNP) were assessed after stimulation with either the 34 pools of 701 overlapping peptides covering the regions of HIV-1 Env, Pol, Gag, Vif, Nef, Tat or some single peptides, by using various assays including enzyme-linked immunospot (ELISPOT) and CFSE Carboxy-fluorescein diacetate, succinimidyl ester (CFSE) labeling and flow cytometry.</p><p><b>RESULTS</b>HIV-1 Gag peptides induced the highest frequency of IFN-gamma secreting cells, followed by Nef, Tat, and Vif. Meanwhile, Env and Pol failed to induce significant responses. In the IFN-gamma ELISPOT assay, stimulation with single peptide and the corresponsive peptide pool generated analogous results. In addition, the frequencies of IFN-gamma secreting cells and the proliferation percentage of CD8+ T cells detected-ELISPOT and CFSE labeling and flow cytometry were proportional, when single peptides were used for stimulation.</p><p><b>CONCLUSION</b>CD8+ T cells can respond to some specific HIV-1 epitopes and induce immunodominant responses. As a complimentary approach to the standard of ELISPOT assay, We recommend a novel CFSE labeling and flow cytometry assay for the examination of immunodominance in studies of HIV-1 specific proliferation percentage of CD8+ T cell responses.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Virology
/
Enzyme-Linked Immunosorbent Assay
/
HIV Infections
/
Immunodominant Epitopes
/
HIV-1
/
Interferon-gamma
/
CD8-Positive T-Lymphocytes
/
Allergy and Immunology
/
Metabolism
Limits:
Adult
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2007
Type:
Article
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