Preliminary analyses for influence of mutant PrPs with different number of octapeptide / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 208-210, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-248802
ABSTRACT
<p><b>OBJECTIVE</b>The present study was conducted to understand the effects of PrP in different octapeptide repeats on proliferation of HeLa cells.</p><p><b>METHODS AND RESULTS</b>Mutant PrPs with octapeptide repeat insertion were transiently expressed in HeLa cells and their results of MTT assay showed stronger cytotoxic effect on the proliferation of cells than wild-type PrP. Annexin V/PI assay also demonstrated that the expression of mutant PrPs was much easier to induce apoptosis than wild-type in HeLa cells. The percentage of both early and late stage apoptosis in mutant groups were significantly higher than that of wild type.</p><p><b>CONCLUSION</b>These data suggest that the expression of mutant PrPs associated with familial CJD is much easier to induce apoptosis in cultured cells than expression of wild type PrP.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oligopeptides
/
Physiology
/
Plasmids
/
Prions
/
HeLa Cells
/
Transfection
/
Cell Survival
/
Blotting, Western
/
Apoptosis
/
Colorimetry
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2007
Type:
Article
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