Development of an enzyme-linked immunosorbent assay for the diagnosis of lethal avian influenza virus infection / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 180-181, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-248809
ABSTRACT
<p><b>OBJECTIVE</b>To establish a convenient method for diagnosis of lethal avian influenza virus infection.</p><p><b>METHODS</b>Ninety-six-well microtiter plates were coated with 100 microl of H5N1 protein (0.1 microg/ml) diluted in coating buffer. These plates were used for enzyme-linked immunosorbent assay (ELISA).</p><p><b>RESULTS</b>Four of the 23 suspected borderline cases of lethal avian influenza virus infection were positive by the ELISA, and all the healthy controls (n=234) were negative. These results were confirmed by hemagglutination-inhibition (HI) and neutralization tests, and results were totally consistent (100 percent) with each other among the three methods.</p><p><b>CONCLUSION</b>The ELISA may be used in the screening for lethal avian influenza virus infection.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Enzyme-Linked Immunosorbent Assay
/
Diagnosis
/
Allergy and Immunology
/
Influenza, Human
/
Influenza A Virus, H5N1 Subtype
/
Methods
/
Antibodies, Viral
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2007
Type:
Article
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