Transduction of dendritic cells by recombinant adeno-associated virus (rAAV) carrying hepatitis B virus antigens / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 105-107, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-248834
ABSTRACT
<p><b>OBJECTIVE</b>To test the efficiency of infection of recombinant adeno-associated virus (rAAV) carrying hepatitis B virus S, C or X antigen, rAAV-HBV-S, C, X to human dendritic cells.</p><p><b>METHODS</b>Recombinant AAV plasmids containing HBV-S, C or X gene were constructed and packaged into rAAV in 293 cells. Monocytes were isolated from healthy donor and pulsed by rAAV-HBV-S, X, C or 293 lysate as control at the first day of isolation, then the dentritic cells were cultured for 7 days in vitro. The transcription and expression of HBV-S, C or X gene were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) or intracellular staining with fluorescence activated cell sorter (FACS), respectively.</p><p><b>RESULTS</b>The titer of rAAV-HBV-S, C, X virus was approximately 10(-7) copies per ml. After infection the HBV-S, C or X transcription expression could be seen by RT-PCR in the infected dendritic cells, the efficiency was about 90 percent by FACS.</p><p><b>CONCLUSION</b>rAAV-HBV-c can effectively infect and pulse dendritic cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transduction, Genetic
/
Virology
/
Dendritic Cells
/
Gene Expression
/
Cell Line
/
Cells, Cultured
/
Dependovirus
/
Cell Biology
/
Genetic Vectors
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2007
Type:
Article
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