Application of interphase fluorescence in situ hybridization in the diagnosis of lymphoma / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 733-738, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-249052
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the feasibility of interphase FISH in the routine clinicopathological practice and its values in the differential diagnosis of lymphomas.</p><p><b>METHODS</b>A total of 74 fresh tissue samples clinically suspicious of lymphoma were investigated by FISH using three probes including IgH/bcl-2, IgH/CCND1 and API2/MALT1, corresponding the translocation t(14;18), t(11;14) and t(11;18) respectively. The results of FISH were analyzed and compared with the histopathologic diagnosis.</p><p><b>RESULTS</b>Histological evaluation eventually confirmed that there were 62 cases of lymphoma and 12 cases of reactive lymphoid processes. The translocations were detected in 7 cases in 62 cases of lymphoma 3 demonstrated t(14;18) including 2 cases of follicular lymphomas and 1 nodular sclerosing Hodgkin lymphoma. Four cases had t(11;14) including mantle cell lymphoma (2 cases), follicular lymphoma (1 case) and small cell lymphoma (1 case). A lymphoid hyperplasia case showed detectable t(14;18). All 25 cases of DLBCL showed no evidence of t(14;18). Amplification or loss of regional genes was seen more often in malignant than in the benign cases.</p><p><b>CONCLUSION</b>Interphase FISH offers useful ancillary technology that plays an important role in differential diagnosis and classification of lymphoma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Translocation, Genetic
/
Chromosomes, Human, Pair 11
/
Chromosomes, Human, Pair 14
/
Chromosomes, Human, Pair 18
/
Lymphoma, Non-Hodgkin
/
Hodgkin Disease
/
Leukemia, Lymphocytic, Chronic, B-Cell
/
Lymphoma, Follicular
/
Lymphoma, Large B-Cell, Diffuse
Type of study:
Diagnostic study
Limits:
Adolescent
/
Adult
/
Aged
/
Aged80
/
Child
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2009
Type:
Article
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