Effect of hydrogen sulfide on human colon cancer SW480 cell proliferation and migration in vitro / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of hydrogen sulfide (H2S) on the proliferation and migration of human colon cancer SW480 cells and explore its molecular mechanisms.</p><p><b>METHODS</b>The proliferation of SW480 cells exposed to different concentrations of NaHS for varying time lengths was analyzed by MTT assay, and the changes in cell migration was evaluated using wound-healing assay. The changes in the expression levels of MMP-2, MMP-9 and SIRT1 protein were detected by Western blotting in the exposed cells.</p><p><b>RESULTS</b>Compared with the control cells, SW480 cells exposed to 50, 100, 200, or 400 µmol/L NaHS for 24, 48 and 72 h all showed increased proliferative activity. NaHS treatment at 100 µmol/L significantly promoted the cell migration (P<0.01) and enhanced the cellular expressions of MMP-2 (P<0.05) and MMP-9 (P<0.01) proteins; NaHS exposure (100 µmol/L) also resulted in up-regulation of SIRT1 expression in SW480 cells.</p><p><b>CONCLUSIONS</b>H2S can promote proliferation and migration of SW480 cells in vitro, the mechanism of which may involve up-regulated expression of SIRT1.</p>