The effects of embryonic bodies on differentiation of mouse embryonic stem cells to neuronal cells in vitro / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 620-624, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-249543
ABSTRACT
This study was directed at assessing the effects of embryonic bodies on differentiation of the embryonic stem cells. The embryonic bodies of different days gained by means of hanging, suspending and hung-suspended were processed by all-trans retinotic acids for 4 days. Then they were detected by immunocytochemistry methods and were measured to detect the fluorescence intensity changes of calcium after Glu stimulation. During this period, we observed the differentiation process and compared the differentiation ratio of embryonic stem cells processed by means of hanging, suspending for several days. It was found that the differentiation ratio of EBs obtained by suspending for 3 days, or by hanging for 3 days and suspending for 1 day, is relatively higher, which can help us find out the intrinsic differentiation mechanism of embryonic stem cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Stem Cells
/
Tretinoin
/
Cell Differentiation
/
Cells, Cultured
/
Cell Biology
/
Embryo, Mammalian
/
Embryonic Induction
/
Mice, Inbred ICR
Limits:
Animals
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2006
Type:
Article
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