Research on preparation of silk fibroin and its biocompatibility with rat bone marrow mesenchymal stem cells / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 560-564, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-249556
ABSTRACT
The newly developed approach of tissue engineering has been shown to be great potential on the ligament reconstruction, however, the criterion for the scaffolding material was strict. The scaffold material must have enough strength as well as elasticity, at the same time, it should be biocompatible. As a nature protein, silk is a promising tissue engineering scaffold material for its excellent mechanical property. However, because of the contamination of sericin, the chief problem of silk's medical use is degumming. We compared three degumming reagents to choose the one which has least effect on the mechanical property of silk, and then the best degumming condition was confirmed 0.4%NazCO3, 90 degrees C, 1 h. Rat bone morrw mesenchymal stem cells (rMSCs) were seeded on the fibroin, and scanning electron microscope (SEM) and fluorescence microscope were used to detect the biocompatibility of it. And the results showed that fibroin had outstanding biocompatibility and cell affinity, which indicated the further use of fibroin in tissue engineering.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Biocompatible Materials
/
Bone Marrow Cells
/
Cells, Cultured
/
Chemistry
/
Cell Biology
/
Tissue Engineering
/
Silk
/
Mesenchymal Stem Cells
/
Fibroins
Limits:
Animals
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2006
Type:
Article
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