Influence of biomateriai on focal adhesion kinase of platelet: ex vivo study / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 350-352, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-249602
ABSTRACT
This study was aimed to detect the biomaterial-induced phosphorylation level of platelet focal adhesion kinase, a key component of signal transduction pathways. Flat tubings, made from 5 biomaterials organotin-PVC, PU50, PU60, PU70, and PTFE,were connected to an ex vivo flow circuit with canine femoral artery and vein. After 5 min, the adherent platelets on the inner surface of the tubing were lysed, then FAK and phospho-FAK were examined, using immunoprecipitation and Western blot. The results showed a significant increase in the FAK value on the PVC and PTFE surface,compared to the PUs; among the PUs, the FAK value decreased in the following order PU70/PU60/PU50. The phospho-FAK value on the five groups of surface decreased in the following order PVC/PTFE/PU70, PU60, PU50; there was no significant difference among the PUs with regard to the phospho-FAK value. In conclusion, after platelet-material interaction, biomateriais may lead to the phosphorylation of FAK of platelets;the level of phosphorylation of FAK has been positively linked to the thrombogenicity of materials; the phospho-FAK value may be a valid molecular parameter of the haemocompatibility assessment; the FAK value may supply references to the haemocompatibility assessment.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphorylation
/
General Surgery
/
Biocompatible Materials
/
Blood Platelets
/
Materials Testing
/
Chemistry
/
Focal Adhesion Protein-Tyrosine Kinases
/
Femoral Artery
/
Femoral Vein
Limits:
Animals
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2006
Type:
Article
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