Influential factor analysis and improvement of cultural method of oral mucosal epithelial cells / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To analyze influential factors of cultural method of human oral mucosal epithelial cells (hOMEC)and to establish a reliable cell culture system for hOMEC.</p><p><b>METHODS</b>Benzalkonium bromide and gentamicin sulfate were used to prevent microbial contamination. Separations of epithelium from underlying connective tissues with Dispase at different concentration were compared. Enzyme digestion was used to isolate cells and keratinocyte-serum free medium(K-SFM) was employed for primary culture and subculture of hOMEC.</p><p><b>RESULTS</b>Microbial contamination was under control. Separation of epithelium from underlying connective tissues with 0.40% Dispase was more complete than that of 0.25% Dispase. The cells grew fast and well in vitro.</p><p><b>CONCLUSION</b>The high successful culture of hOMEC and simplified procedures could be obtained with improvement of methods.</p>