Insulin expression in rats exposed to cadmium / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 295-301, 2007.
Article
in English
| WPRIM
| ID: wpr-249852
ABSTRACT
<p><b>OBJECTIVES</b>To investigate the effects of cadmium exposure on insulin expression in rats.</p><p><b>METHODS</b>Eighteen adult SD rats were administered cadmium subcutaneously (0.5, 1.0, and 2.0 mg/kg x bw). The effects on endocrine of pancreas were assessed. The levels of cadmium and zinc in pancreas, blood and urine glucose, serum insulin and urine NAG (N-acyetyl-beta-glucosaminidase) were determined. The gene expressions of metallothionein (MT) and insulin were also measured, and the oral glucose tolerance tests (OGTT) were carried out.</p><p><b>RESULTS</b>The contents of cadmium in pancreas in cadmium-treated rats were higher than that in the control group, which was associated with slight increase of zinc in pancreas. Cadmium-exposed rats (1.0 and 2.0 mg/kg x bw) demonstrated a marked glucose intolerance. But the levels of serum insulin did not change significantly after cadmium administration, and the UNAG had no change in Cd-treated group. The gene expression of insulin decreased in 1.0 and 2.0 mg/kg x bw cadmium-exposed groups, compared with the control group. The expression of MT-I was higher in the groups exposed to 1.0 and 2.0 mg/kg x bw cadmium while the expression of MT-II was higher in the group exposed to 2.0 mg/kg x bw cadmium.</p><p><b>CONCLUSIONS</b>Cadmium may be accumulated in the pancreas, resulting in the change of the expression of insulin, MT-I and MT-II genes. Cadmium can influence the biosynthesis of insulin, but does not induce the release of insulin. The dysfunction of pancreas occurs earlier than that of kidney after administration of cadmium.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Urine
/
Blood
/
Blood Glucose
/
Cadmium
/
Base Sequence
/
Gene Expression
/
Rats, Sprague-Dawley
/
DNA Primers
/
Reverse Transcriptase Polymerase Chain Reaction
/
Toxicity
Limits:
Animals
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2007
Type:
Article
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