Establishment and optimization of the regeneration system for common dandelion (Taraxacum officinale Weber) / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 244-249, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-249917
ABSTRACT
A protocol is presented for direct and indirect regeneration of common dandelion (Taraxacum officinale Weber) from leaf and petiole explants. Multiple shoots were obtained on MS medium containing 0.2 mg/L IAA and 1 mg/L TDZ. For indirect regeneration, fragile calli were obtained from leaf and petiole explants on MS medium supplemented with 0.5 mg/L 2,4-D and 2.0 mg/L 6-BA. Regenerated plantlets were obtained when these calli were cultured on MS medium containing 1.0 mg/L 6-BA. Random amplified polymorphic DNA (RAPD) analysis of nine regenerated plantlets revealed 61 scorable bands from 10 primers, including three specific bands.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Regeneration
/
Plant Leaves
/
Germination
/
Culture Media
/
Taraxacum
/
Tissue Culture Techniques
/
Methods
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2005
Type:
Article
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