Humoral immune response elicited by plasmid DNA containing HGV E2 gene fragment / 生物工程学报
Chinese Journal of Biotechnology
; (12): 683-688, 2004.
Article
in En
| WPRIM
| ID: wpr-249954
Responsible library:
WPRO
ABSTRACT
In order to study the feasibility of E2 gene fragment of hepatitis virus G(HGV) as a component of DNA vaccine against the hepatitis virus G infection, a 559bp DNA fragment encoding HGV E2 was cloned into plasmid pCMV-S from pThioHis-E2 in the same reading frame with HBsAg gene to form a recombinant plasmid named pCMV-S-E2. BALB/c mice of Kunming strain were immunized with purified plasmid DNA of pCMV-S-E2 by intra-muscularly inoculation. The immunizations were boosted twice at an interval of 14 days. The whole blood was collected from mice orbit on the day-8 after the last boost. Mice sera were screened by ELISA to determine the humoral immune response using E2-GST fusion protein as the immobilized antigen and the sera from mice immunized with pCMV-S as control. The result indicated that the immunization with plasmid DNA of pCMV-S-E2 could induce quite strong humoral immune response.
Full text:
1
Index:
WPRIM
Main subject:
Plasmids
/
Blood
/
Recombinant Fusion Proteins
/
Viral Hepatitis Vaccines
/
Hepatitis Antibodies
/
Viral Envelope Proteins
/
Vaccines, DNA
/
GB virus C
/
Allergy and Immunology
/
Genetics
Limits:
Animals
Language:
En
Journal:
Chinese Journal of Biotechnology
Year:
2004
Type:
Article