Isolation of thermophilic bacteria Thermus sp. YBJ-1 and cloning of amylase gene / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 434-436, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-249968
ABSTRACT
Thermophilic bacteria strain YBJ-1 was isolated from hot spring samples collected from Yangbajing, Tibet. The 16sr DNA sequence of YBJ-1 (1511bp in length) shares 98% identity with that of Thermus scotoductus strain ITI-252T. The full-length ORF of amylase gene of YBJ-1 (amyT) was amplified by PCR technique and cloned into T-vector. The complete sequence of amyT is 1767bp in length, coding for 588 amino acids. The deduced amino acids share 99% similarity with alpha-cyclodextrinse of Bacillus sterothermophilus, 96% with maltogenic amylse of Thermus. sp IM6501, and 81% with neopullulanase of Bacillus sterothermophlus.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Thermus
/
Bacterial Proteins
/
Open Reading Frames
/
Cloning, Molecular
/
Culture Media
/
Genetics
/
Amylases
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2004
Type:
Article
Similar
MEDLINE
...
LILACS
LIS