Enhancements in ethanol tolerance of a self-flocculating yeast by calcium ion through decrease in plasmalemma permeability / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 715-719, 2003.
Article
in English
| WPRIM
| ID: wpr-250002
ABSTRACT
Ca2+ at 1.64 mmol/L markedly increased ethanol tolerance of a self-flocculating fusant of Schizosaccharomyces pombe and Saccharomyces cerevisiae. After 9 h of exposure to 20% (V/V) ethanol at 30 degrees C , no viability remained for the control whereas 50.0% remained for the cells both grown and incubated with ethanol in Ca2+ -added medium. Furthermore, when subjected to 15% (V/V) ethanol at 30 degrees C, the equilibrium nucleotide concentration and plasma membrane permeability coefficient (P' ) of the cells both grown and incubated with ethanol in Ca2+ -added medium accounted for only 50.0% and 29.3% those of the control respectively, indicating that adding Ca2+ can markedly reduce plasma membrane permeability of yeast cells under ethanol stress as compared with the control. Meanwhile, high viability levels acquired by the addition of Ca2+ exactly corresponded to the striking decreases in extracellular nucleotide concentration and P' achieved with identical approach. Therefore, the enhancing effect of Ca2+ on ethanol tolerance of this strain is closely related to its ability to decrease plasma membrane permeability of yeast cells subjected to ethanol stress.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Saccharomyces cerevisiae
/
Schizosaccharomyces
/
Temperature
/
Cell Membrane
/
Cell Membrane Permeability
/
Calcium
/
Ethanol
/
Metabolism
Language:
English
Journal:
Chinese Journal of Biotechnology
Year:
2003
Type:
Article
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