The relationship between microRNA-18 and BTG2 in the carcinogenesis of hepatocellular carcinoma / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 42-45, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-250061
ABSTRACT
<p><b>OBJECTIVE</b>To study the difference of microRNA expression between HepG2 cells and L02 cells, and to identify the target genes of microRNA-18 (miR-18).</p><p><b>METHODS</b>The differentially expressed miRNAs between HepG2 cells and L02 cells were identified by miRNA chip. Target genes of miR-18 were predicted bioinformatically. Furthermore, the expression of B-cell translocation gene 2 (BTG2), a putative target gene of miR-18, was analyzed in hepatocellular carcinoma tissues and the surrounding non-cancerous tissues by RT-PCR and northern blot.</p><p><b>RESULTS</b>miR-18 was over-expressed in HepG2 cells compared to L02 cells. Altogether 609 genes, including genes involved in cell proliferation, differentiation, apoptosis and transcriptional regulation, are identified as putative miR-18 targets. The mRNA level of BTG2 was much lower in hepatocellular carcinoma tissues than in the corresponding non-cancerous tissues.</p><p><b>CONCLUSION</b>miR-18 is over-expressed in HepG2 cells compared to L02 cells, and it may negatively regulate the expression of BTG2, a tumor suppressor gene.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Gene Expression Regulation, Neoplastic
/
Cells, Cultured
/
Apoptosis
/
Immediate-Early Proteins
/
Carcinoma, Hepatocellular
/
Reverse Transcriptase Polymerase Chain Reaction
/
Gene Expression Profiling
/
Cell Biology
/
Tumor Suppressor Proteins
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2009
Type:
Article
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