Growth factors may enhance c-fos and c-jun gene expressions of hepatic stellate cells / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 902-904, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-250086
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of growth factors on c-fos and c-jun gene expressions in hepatic stellate cells.</p><p><b>METHODS</b>Hepatic stellate cell (HSC) T6 was cultured in media containing different concentrations of platelet derived growth factor (PDGF) (8 ng/ml, 40 ng/ml or 200 ng/ml) and transforming growth factor (TGF) beta (0.2 ng/ml, 1 ng/ml or 5 ng/ml) and the cells were collected at different incubation periods (8, 24, 48 or 72 h). Total RNA of the HSC was isolated and c-fos and c-jun gene expression levels were measured by reverse transcription polymerase chain reaction.</p><p><b>RESULTS</b>C-fos gene expression levels of the HSC cultured in low (8 ng/ml), medium (40 ng/ml) and high (200 ng/ml) concentrations of PDGF were all much higher than those of the control group after exposure to PDGF at 8, 24, 48 or 72 h. The c-fos gene expression levels of the HSC increased as the dosage of PDGF increased and there were significant differences of c-fos gene expression among the three PDGF groups. C-jun gene expression levels of the HSC in low (0.2 ng/ml), medium (1.0 ng/ml) and high (5.0 ng/ml) concentrations of TGF beta groups were much higher than those of the control group after exposure to TGF beta at 8, 24, 48 or 72 h. The c-jun gene expression levels of the HSC increased as the dosage of TGF beta increased and there were significant differences of c-jun gene expression among the three TGF beta groups.</p><p><b>CONCLUSION</b>PDGF and TGF beta can strongly up-regulate c-fos and c-jun gene expressions in hepatic stellate T6 cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Platelet-Derived Growth Factor
/
Gene Expression
/
Cells, Cultured
/
Transforming Growth Factor beta
/
Proto-Oncogene Proteins c-jun
/
Proto-Oncogene Proteins c-fos
/
Hepatic Stellate Cells
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2008
Type:
Article
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