The Expression of TGF-beta Isoform mRNA in the Cataract Lens Epithelial Cell
Journal of the Korean Ophthalmological Society
;
: 1393-1400, 2005.
Article
in Korean
| WPRIM
| ID: wpr-25011
ABSTRACT
PURPOSE:
Transforming growth factor (TGF)-beta is recognized as being associated with cataractogenesis. We quantitated the mRNA expression of TGF-beta isoforms in cataractous crystalline lens to determine the effect of the isoforms on cataractogenesis.METHODS:
With lens epithelial cells from thirty eyes in thirty patients, the mRNA expressions of TGF-beta1, beta2 and beta3 were quantitated by real-time SYBR Green polymerase chain reaction and the results were compared according to cataract type and presence of diabetes mellitus.RESULTS:
Each isoform mRNA of TGF-beta was expressed TGF-beta3 in all 30 eyes, TGF-beta1 in 29 eyes (96.7%), with the exception being one diabetic senile cataract, and TGF-beta2 in 9 eyes. The amount of TGF-beta1 mRNA expression was significantly lower in the diabetic cataracts than in the non-diabetic cataracts (P=0.056).CONCLUSIONS:
TGF-beta was associated with cataractogenesis. It is significant that the expression of TGF-beta2 mRNA was decreased in all cataracts. The decrease of TGF-beta1 mRNA expression was more meaningful in the diabetic cataracts than in the non-diabetic cataracts.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cataract
/
RNA, Messenger
/
Transforming Growth Factors
/
Polymerase Chain Reaction
/
Transforming Growth Factor beta
/
Protein Isoforms
/
Diabetes Mellitus
/
Epithelial Cells
/
Transforming Growth Factor beta1
/
Transforming Growth Factor beta2
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2005
Type:
Article
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