Effects of hepatectomized rat serum on the transdifferentiation of adult rat bone marrow cells into hepatocyte-like cells / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 730-733, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-250111
ABSTRACT
<p><b>OBJECTIVES</b>To study the effects of hepatectomized rat serum and hepatocyte growth factor (HGF) on the transdifferentiation of adult rat bone marrow stem cells (ABMSCs) into hepatic parenchymal cells.</p><p><b>METHODS</b>The serum was collected from the rats 24 hours after being subjected to subtotal hepatectomy. ABMSCs were collected and cultured in DMEM/F12 (11) containing the hepaetectomized rat serum or HGF. The differentiated hepatocyte-like cells were labeled with CM-DiI and administrated by tail vein injection into the isogeneic rats. The cultured and injected cells were both identified by immunocytochemistry and cultured cells were assayed using RT-PCR and Western blot.</p><p><b>RESULTS</b>Hepatectomized rat serum and HGF were demonstrated to have the effect of inducing transdifferentiation of ABMSCs into hepatocyte-like cells in vitro. The differentiated cells expressed albumin mRNA and albumin after 7 days++'s co-incubation. Albumin-expressing and CM-DiI positive hepatocyte-like cells were characterized in livers and spleens of the rats injected with the cultivated cells.</p><p><b>CONCLUSION</b>ABMSCs could transdifferentiate into hepatic parenchymal cells by hepatectomized rat serum or HGF.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Stem Cells
/
Bone Marrow Cells
/
Cell Differentiation
/
Cells, Cultured
/
Rats, Sprague-Dawley
/
Hepatocyte Growth Factor
/
Culture Media
/
Hepatocytes
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2004
Type:
Article
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