The anti-cancer effect of siRNA targeting human telomerase reverse transcriptase in SMMC-7721 cells / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the anti-cancer effects of siRNAs targeting hTERT in SMMC-7721 cells.</p><p><b>METHODS</b>Two siRNAs targeting hTERT mRNA were designed and synthesized by T7 transcription system in vitro. MMT, RT-PCR and Western blot were applied to evaluate effects on inhibiting cell growth, hTERT mRNA and protein expression in SMMC-7721 cells.</p><p><b>RESULTS</b>siRNAs decreased cell proliferation in a dose-dependent manner. At a concentration of 100 nmol/L, siRNAs exhibited obvious effects on inhibiting hTERT mRNA and protein expression in SMMC-7721 cells.</p><p><b>CONCLUSION</b>siRNAs targeting hTERT have significant inhibitory effects on hTERT gene expression in SMMC-7721 cells. siRNA has the possibility to become a new anti-cancer agent</p>