Establish a screening system for selection of mRNA target sites for HBsAg to construct siRNA with shRNA / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 515-518, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-250170
ABSTRACT
<p><b>OBJECTIVE</b>To find some effective short interfering RNA's sites targeting HBV surface gene sequence using shRNA expression vectors.</p><p><b>METHODS</b>Four shRNA expression vectors targeting HBV surface gene sequence were constructed based on pAVU6 + 27 vector, and cotransfected into AD293 cells with HBs-EGFP fusion gene plasmid. The changes of HBs-EGFP image were detected by FACS and microscopy. The HBs-EGFP mRNA expression was evaluated by RT-PCR.</p><p><b>RESULTS</b>Four shRNA expression vectors and HBs-EGFP fusion gene plasmid were successfully constructed. pAVU6 + 4sh579 vector inhibited the HBs-EGFP expression by 69.8% in AD293 and suppressed the HBs-EGFP mRNA expression by 74.6%.</p><p><b>CONCLUSIONS</b>The results showed that the 579 site of HBV surface gene sequence was an effective target and pAVU6 + 4sh579 vector could suppress the HBs-EGFP expression in AD293 cells</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Gene Expression Regulation, Viral
/
Gene Targeting
/
Gene Silencing
/
RNA-Induced Silencing Complex
/
RNA, Small Interfering
/
RNA Interference
/
Genetics
/
Hepatitis B Surface Antigens
/
Metabolism
/
Methods
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2004
Type:
Article
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