Preparation and identification of Jujuboside A artificial antigen / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1880-1883, 2016.
Article
in Zh
| WPRIM
| ID: wpr-250473
Responsible library:
WPRO
ABSTRACT
Immunogenic antigen (jujuboside A-BSA) and coating antigen (jujuboside A-OVA) of jujuboside A were synthesized by sodium periodate oxidation method for the first time. Jujuboside A artificial antigen was confirmed by matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF-MS). The titer and specificity of the antibody in serum of immunized mice were detected by enzyme-linked immunosorbent assay (ELISA). The corrected relation curve of inhibition rate showed that the antibody against Jujuboside A obtained from immunized mice could bind to jujuboside A and the titer was up to 1∶4 000. The jujuboside A artificial antigen was synthesized, which can be used further to preparation of monoclonal antibody and the pharmacokinetics study of jujuboside A in laboratory animals.
Full text:
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Index:
WPRIM
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2016
Type:
Article