Molecular characterization of hantavirus Shandong isolate JNL virus strain / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 112-115, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-250528
ABSTRACT
<p><b>OBJECTIVE</b>To understand the molecular epidemiologic characteristics of hantavirus Shandong isolate JNL virus strain.</p><p><b>METHODS</b>The complete M and S gene of the JNL virus isolated from Shandong Province was amplified by RT- PCR, and the purified PCR product was cloned into T vector for sequencing.</p><p><b>RESULTS</b>The results revealed that the JNL M segment was 3615 bp in length, encoding 1135 amino acids, and the S segment was 1698 bp encoding 429 amino acids, JNL belongs to HTN virus. The comparison of homology with HTN and SEO types showed that the difference of M and S complete sequences between JNL and all other HTN virus strains reached 20.0%-20.6%, and 15.5%-16.0%, respectively. Phylogenetic tree also showed that the position of JNL is located at a different clade.</p><p><b>CONCLUSIONS</b>HTN virus Shandong local isolate JNL strain is a new specific HTN subtype virus.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Virology
/
DNA, Viral
/
Sequence Homology, Nucleic Acid
/
Classification
/
Sequence Homology, Amino Acid
/
Sequence Analysis, DNA
/
Hantaan virus
/
Reverse Transcriptase Polymerase Chain Reaction
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2003
Type:
Article
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