Inhibition of Luciferase expression in mammalian cells by AAV vector plasmid mediated Luciferase shRNA / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 253-255, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-250542
ABSTRACT
<p><b>OBJECTIVE</b>Constructing a plasmid containing the shRNA of luciferase to suppress the expression of luciferase in BHK-21 cell.</p><p><b>METHODS</b>A 334 bp human U6 snRNA promoter was amplified from human genomic DNA by PCR and ligated to a 21 bp reverse repeated motif of luciferase target sequence with 9 bp spacer and AAV plasmid pSNAV. The recombinant pSNAV/U6/Luc plasmid cotransfected with pMAMneoLuc or transfected luciferase cell line to detect the effect of luciferase expression separately.</p><p><b>RESULTS</b>pSNAV/U6/luc suppresses the luciferase expression from pMAMneoLuc by 50% and luciferase cell line by 70%.</p><p><b>CONCLUSIONS</b>The results showed that the short hairpin RNA of luciferase can efficiently suppress its expression in BHK-21.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
RNA
/
Transfection
/
Gene Expression
/
Cell Line
/
Promoter Regions, Genetic
/
Genetic Vectors
/
Genetics
/
Luciferases
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2002
Type:
Article
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