Nucleotide sequence analysis of new genotype of hepatitis G virus in population at high risk for HCV infection in Guangxi / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 242-245, 2002.
Article
in Zh
| WPRIM
| ID: wpr-250545
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To examine the prevalence and the sequence of the genes of new genotypes of hepatitis G virus (HGV) in Guangxi, China.</p><p><b>METHODS</b>Serum samples were collected from 85 intravenous drug abusers (IVDAs), 80 patients with liver diseases (PLDs) and 50 blood donors (BDs). All sera (n=215) were tested by using EIA for HBsAg, anti-HCV and anti-HIV, and by using nested PCR for HGV RNA. In 62 subjects positive for HGV, HGV RNA was sequenced, and a phylogenetic tree was constructed for analyzing genotypes of HGV.</p><p><b>RESULTS</b>HGV RNA was detected in 85 of 215 serum samples (39.53%). The positivity rates for HBsAg, anti-HCV and anti-HIV were 39.07%, 42.79% and 0, respectively. First, 11 nucleotide sequences were determined and the isolates were grouped into three clusters with HGV. 5 of 11 HGV isolates clustered in a distinct phylogenetic branch (genotype Asia) which was different from the described GBV-C and HGV sequences, suggesting the presence of a new genotype of HGV in this locality. Second, 51 nucleotide sequences were determined and analyzed for their genotypes of HGV, and showed genotype GBV-C (3.23%), genotype HGV 30-65% and new genotype (genotype Asia) 64.51%, respectively.</p><p><b>CONCLUSIONS</b>There were subgenotypes in 3 genotypes of HGV; The predominant genotypes of HGV were genotype Asia and genotype HGV among IVDAs, PLDs, and BDs patients in Guangxi, China.</p>
Full text:
1
Index:
WPRIM
Main subject:
Virology
/
Blood Donors
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RNA, Viral
/
Substance Abuse, Intravenous
/
China
/
Polymerase Chain Reaction
/
Epidemiology
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Hepatitis C
/
Sequence Analysis, RNA
/
GB virus C
Type of study:
Etiology_studies
Limits:
Adult
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Female
/
Humans
/
Male
Country/Region as subject:
Asia
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2002
Type:
Article