Proteomic study of the effect of recombinant hFGF-10 adenovirus on HaCat cells / 药学学报
Acta Pharmaceutica Sinica
;
(12): 55-59, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-250620
ABSTRACT
This study is to investigate the effect of recombinant hFGF-10 adenovirus on the proteome of HaCat cells, and to speculate further the possible mechanism of the effect of hFGF-10 on HaCat cells via differentially expressed proteins identified. Two-dimensional gel electrophoresis (2-DE) combined with tandem time-of-flight mass spectrometry was applied to identify the differentially expressed protein spots on the 2-DE maps of the whole-cell proteins from Ad infected and rAd-hFGF-10 infected HaCat cells. The mRNA and protein levels of the differentially expressed proteins were confirmed with semi-quantitative RT-PCR and Western blotting. The results showed that the 2-DE maps with high resolution were obtained, and four selected differentially expressed proteins involved in cell apoptosis, cytoskeleton regulation and protein degradation were identified with MALDI-TOF/TOF. The mRNA and protein levels of one of the differentially expressed proteins, VDAC2, were up-regulated in HaCat cells infected with the recombinant hFGF-10 adenovirus. The differentially expressed protein, VDAC2, may be related to the bioactivities of hFGF-10.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombinant Proteins
/
RNA, Messenger
/
Transfection
/
Electrophoresis, Gel, Two-Dimensional
/
Keratinocytes
/
Up-Regulation
/
Cell Line
/
Adenoviridae
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Cell Biology
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2010
Type:
Article
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