RP-HPLC determination of loganin in mouse plasma and its pharmacokinetics / 药学学报
Acta Pharmaceutica Sinica
;
(12): 548-550, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-251104
ABSTRACT
<p><b>AIM</b>To develop a method for determination of loganin in mouse plasma by using high-performance liquid chromatography. The method was employed to study pharmacokinetics of loganin.</p><p><b>METHODS</b>An RP-C18 was used as the stationary phase. The mobile phase consisted of methanol-water (3070), at the flow-rate of 0.8 mL.min-1. The UV absorbance detector was set at 240 nm. Plasma samples were treated with solid phase extraction.</p><p><b>RESULTS</b>The recovery of loganin in mouse plasma was 86.0%-91.5%. The calibration curve in plasma was linear over the range of 0.01-5.00 micrograms.mL-1. The limit of quantitation was 10 ng.mL-1. The RSDs of intra-day and inter-day (n = 5) were less than 15%. The pharmacokinetic parameters were Cmax = 6.8 micrograms.mL-1, Tmax = 30 min, T1/2 alpha = 26.1 min, T1/2 beta = 29.01 min.</p><p><b>CONCLUSION</b>The method is accurate, sensitive and suitable for pharmcokinetic study of loganin. The absorption and elimination of loganin were rapid after ig in mice.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Pharmacokinetics
/
Adjuvants, Immunologic
/
Chromatography, High Pressure Liquid
/
Area Under Curve
/
Iridoids
/
Methods
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2002
Type:
Article
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