HPLC-fluorescent spectrometric determination of serum mexiletine concentration after derivatization with fluram / 药学学报

ABSTRACT

<p><b>AIM</b>To establish an HPLC-fluorescent spectrometric method for the determination of mexiletine hydrochloride in plasma after derivatization with fluram.</p><p><b>METHODS</b>Fluram acetone solution was added to the deproteinized plasma with acetone to obtain the derivative of mexiletine. The HPLC method was performed on a column of Allitima C18 (150 mm x 4.6 mm, 5 microns) with the mobile phase of methanol-water-diethylamine-phosphoric acid buffer (2.4 mol.L-1, pH 4.0) (70282), and the detective wavelength were set at Ex 392 nm and Em 480 nm.</p><p><b>RESULTS</b>Mexiletine has a liner range over the concentration range from 0.100-6.400 mg.L-1. The lowest detectable concentration of this method was 5 micrograms.L-1 (S/N > or = 4). The intra-day and inter-day RSDs were 1.34%-5.31%, respectively.</p><p><b>CONCLUSION</b>This method is simple, selective and can be used for therapeutic drug monitoring (TDM) and pharmacokinetic studies of mexiletine.</p>