Differential protein analysis in rat renal proximal tubule epithelial cells in response to acetazolamide and its relation with the inhibition of AQP1 / 药学学报
Acta Pharmaceutica Sinica
;
(12): 169-172, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-251150
ABSTRACT
<p><b>AIM</b>To study the endogenous mechanism for the inhibition of aquaporin-1 expression in rat renal proximal tubule epithelial cells in response to acetazolamide.</p><p><b>METHODS</b>Primary cultured rat renal proximal tubule epithelia cells were divided into two groups one was subjected to 1 x 10(-5) mol.L-1 acetazolamide, the other served as normal control. When grown to sub-confluency, the cells were disintegrated to perform isoelectrofocusing electrophoresis in order to find the differential proteins induced by the acetazolamide treatment. The differential proteins were defined by peptide mass fingerprinting technology.</p><p><b>RESULTS</b>Two differential proteins were found in the cell disintegrant. The pI 3.8 protein was reduced after treatment, which showed 21.4% similarity with the brush border membrane myosin from rat brain and testis, and 27% with glycogen phosphorylase; The pI 5.5 protein was increased on the contrary, with 20% similarity to phosphatidylinositol transfer protein alpha isoform.</p><p><b>CONCLUSION</b>Acetazolamide inhibited AQP1 expression probably by affecting the expression of pI 3.8 and pI 5.5 proteins.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Peptide Mapping
/
Rats, Sprague-Dawley
/
Aquaporins
/
Cell Biology
/
Diuretics
/
Epithelial Cells
/
Aquaporin 1
/
Isoelectric Focusing
/
Kidney Tubules, Proximal
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2003
Type:
Article
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